General Information

  • ID:  hor001000
  • Uniprot ID:  P06307
  • Protein name:  Cholecystokinin-8
  • Gene name:  CCK
  • Organism:  Homo sapiens (Human)
  • Family:  Gastrin/cholecystokinin family
  • Source:  Human
  • Expression:  NA
  • Disease:  Diseases associated with CCK include Cholecystitis and Biliary Dyskinesia.
  • Comments:  NA
  • Taxonomy:  Homo (genus), Homininae (subfamily), Hominidae (family), Hominoidea (superfamily), Catarrhini (parvorder), Simiiformes (infraorder), Haplorrhini (suborder), Primates (order), Euarchontoglires (superorder), Boreoeutheria, Eutheria, Theria, Mammalia (class), Amniota, Tetrapoda, Dipnotetrapodomorpha, Sarcopterygii (superclass), Euteleostomi, Teleostomi, Gnathostomata, Vertebrata, Craniata (subphylum), Chordata (phylum), Deuterostomia, Bilateria, Eumetazoa, Metazoa (kingdom), Opisthokonta, Eukaryota (superkingdom), cellular organisms
  • GO MF:  GO:0005179 hormone activity; GO:0005184 neuropeptide hormone activity; GO:0005515 protein binding; GO:0051428 peptide hormone receptor binding
  • GO BP:  GO:0001764 neuron migration; GO:0007165 signal transduction; GO:0007409 axonogenesis; GO:0007586 digestion; GO:0042755 eating behavior
  • GO CC:  GO:0005576 extracellular region; GO:0005615 extracellular space; GO:0030424 axon

Sequence Information

  • Sequence:  DYMGWMDF
  • Length:  8
  • Propeptide:  MNSGVCLCVLMAVLAAGALTQPVPPADPAGSGLQRAEEAPRRQLRVSQRTDGESRAHLGALLARYIQQARKAPSGRMSIVKNLQNLDPSHRISDRDYMGWMDFGRRSAEEYEYPS
  • Signal peptide:  MNSGVCLCVLMAVLAAGALT
  • Modification:  T2 Sulfotyrosine;T8 Phenylalanine amide
  • Glycosylation:  NA
  • Mutagenesis:  2-2Y->F: Reduces the quantity of secreted CCK8 by 50%.

Activity

  • Function:  This peptide hormone induces gall bladder contraction and the release of pancreatic enzymes in the gut.Binding to CCK-A receptors stimulates amylase release from the pancreas, binding to CCK-B receptors stimulates gastric acid secretion.
  • Mechanism:  NA
  • Cross BBB:  YES
  • Target:  CCKAR
  • Target Unid:  P32238
  • IC50: NA
  • EC50: NA
  • ED50: NA
  • kd: NA
  • Half life: 18 minute; /1080 seconds ( PubMed ID: 6291099 )

Structure

  • Disulfide bond:  NA
  • Structure ID:  AF-P06307-F1(AlphaFold_DB_ID)

  • Structure: (PDB_ID-from https://www.rcsb.org/; AlphaFold_DB_ID-from https://alphafold.ebi.ac.uk/; hordbxxxxxx_AF2.pdb was predicted structure by AlphaFold2; hordbxxxxxx_ESM.pdb was predicted structure by ESMFold)
    •    hor001000_AF2.pdbhor001000_ESM.pdb

Physical Information

Mass: 118924 Formula: C49H61N9O14S2
Absent amino acids: ACEHIKLNPQRSTV Common amino acids: DM
pI: 3.49 Basic residues: 0
Polar residues: 2 Hydrophobic residues: 2
Hydrophobicity: -37.5 Boman Index: -663
Half-Life: 1.1 hour Half-Life Yeast: 3 min
Half-Life E.Coli: >10 hour Aliphatic Index 0
Instability Index: 9927.5 Extinction Coefficient cystines: 6990
Absorbance 280nm: 998.57

Literature

  • PubMed ID:  6291099
  • Title:  NA